Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2018) [Effective from January 1, 2018]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here
journals IJCMAS
See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 1078-1084
DOI: https://doi.org/10.20546/ijcmas.2018.711.125


Morphological Transformation and Estimation of Normal Growth Curve of Both Morphological Forms of Sporothrix schenckii
Rajesh Kumar Shah1*, Hemant B. Gadekar1, Vikas Jain1, Sweta Kumari2, Apoorva Tripathi1, Sanjo Gupta1 and Neelam1
1Department of Microbiology
2Department of Physiology, RKDFMCH&RC Bhopal- 462026, MP, India
*Corresponding author
Abstract:

Morphological transformation of pathogenic fungi is an important factor contributing in pathogenicity. Sporothrix schenckii is a dimorphic fungus, capable of transforming from filamentous form to yeast form in presence of nutritional factors and environmental conditions. This is very much essential for identification to establish definitive diagnosis of sporotrichosis in-vitro. A six times repeated successive subculture of a standard filamentous S. schenckii (MTCC1359) was done on Brain heart infusion blood agar (BHIBA) incubated at 37°C in presence of 5-10% CO2 and humidity to transform yeast form. A normal growth curve of both yeast and filamentous stage of S. schenckii were plotted by recording every day optical densities (ODs) of broth culture in yeast nitrogen base (YNB) media at 540 nm of wavelength in colorimeter. By observing the graphs of yeast and mould form, the log phase were from 2nd to 14th day and 3rd to 18th day, stationary phase 14th to 20th day and 18th to 21st day, death phase 20th -33rd day and 21st -33rd day respectively. It is necessary to know the normal growth curve of fungus for various further scientific studies which is not standardized yet like standardized bacterial normal growth curve.


Keywords: Morphlogical, Transformation, Sporothrix schenckii, Optical density, Normal growth curve
Download this article as Download

How to cite this article:

Rajesh Kumar Shah, Hemant B. Gadekar, Vikas Jain, Sweta Kumari, Apoorva Tripathi, Sanjo Gupta and Neelam. 2018. Morphological Transformation and Estimation of Normal Growth Curve of Both Morphological Forms of Sporothrix schenckii.Int.J.Curr.Microbiol.App.Sci. 7(11): 1078-1084. doi: https://doi.org/10.20546/ijcmas.2018.711.125