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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 3524-3531
DOI: https://doi.org/10.20546/ijcmas.2018.710.407


Two-Dimensional Gel Electrophoresis of Protein profile from Groundnut (Arachis hypogaea sp. Virginia) at Flower Development Stage
Nimita Umretiya1*, Hitesh Jasani2, Nilam Tulsani1, Abhijeeta Nandhaand B.A. Golakiya1
1Department of Biotechnology, Junagadh Agricultural University, Junagadh-362001, Gujarat, India
2College of Computer, Science and Information Technology,
Junagadh-362015, Gujarat, India
*Corresponding author
Abstract:

Protein is play critical role in flower development. Profiles of total flower development proteins isolated from different stages (Bud, Flower and Peg) peanut cultivar GJG-22, were studied using two-dimensional gel electrophoresis. Protein is extracted by trichloro acetic acid/acetone precipitation method. Comparative analysis was carried out of spots identified using platinum master software at three different flower stages. Protein revealed that total 9193 protein spots with the pH range 4 - 7 and 23-312 kDa range recorded. Out of 9193 spots, maximum 3413 spots were found in pH range 6 - 7 in groundnut samples. Proteomic analysis reveals the translational products of gene expression of plant during flowering stage.


Keywords: Two-Dimensional Electrophoresis (2-DE), Protein profile, Groundnut, Arachis hypogaea, Protein extraction
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How to cite this article:

Nimita Umretiya, Hitesh Jasani, Nilam Tulsani, Abhijeeta Nandha and Golakiya, B.A. 2018. Two-Dimensional Gel Electrophoresis of Protein profile from Groundnut (Arachis hypogaea sp. Virginia) at Flower Development Stage.Int.J.Curr.Microbiol.App.Sci. 7(10): 3524-3531. doi: https://doi.org/10.20546/ijcmas.2018.710.407