Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 3259-3269
DOI: https://doi.org/10.20546/ijcmas.2018.710.377


Bacteriological Profile and Antibiogram of Gram Positive Nosocomial Isolates from Intensive Care Units with Special Reference to Methicillin Resistant Staphylococcus aureus (MRSA) in a Tertiary Care Hospital of North Karnataka, India
Zarrin Afroz1*, Basavaraj C. Metri2, Faisal Nasim Gilani3 and P. Jyothi4
1Department of Microbiology, G.S Medical College and Hospital, Pilkhuwa – 245304 District – Hapur, Uttar Pradesh, India
2Department of Microbiology, 4Department of Forensic Medicine, Shri B.M. Patil Medical College and Research Centre, BLDE University, Vijayapura – 586103, India
3Department of Forensic Medicine, G.S. Medical College and Hospital, Pilkhuwa- 245304, Hapur, India
*Corresponding author
Abstract:

Nosocomial infection is a key factor in determining clinical outcomes among patients admitted in intensive care units. Studies on nosocomial infections in ICUs found that respiratory tract infections, blood stream infections, urinary tract infections and soft tissue infections are the common nosocomial infections in ICUs. These organisms causing nosocomial infections are highly resistant to antibiotics. Early recognition of bacteria and appropriate antimicrobial therapy are essential for controlling infection, preventing the morbidity and improve the quality of life. Aims of this study are to find out the profile of Gram positive bacteria causing nosocomial infection in patients admitted in intensive care units and to know the antibiotic susceptibility pattern of the nosocomial isolates. A total of 192 culture positive samples were taken from various clinical specimens. The organisms were identified by Gram staining, cultural characteristics and a battery of biochemical tests. Drug susceptibility was performed on the isolates by Kirby Bauer’s disk diffusion method. A total of 202 organisms were isolated from 192 various clinical samples. Respiratory tract infection 76(39.6%) was the most common infection in Intensive care units. The number of Gram positive isolates was 60 (29.7%). 142 (70.3%) Gram negative organisms were isolated. The most common Gram positive cocci was Staphylococcus aureus 34 (16.8%) followed by CONS 16(26.6%). Among Gram positive cocci minimum resistance was seen against vancomycin (6.7%) followed by linezolid (10%) and cotrimoxazole (26.6%). Methicillin resistance was seen in 28 (82.35%) of S. aureus. 57.1% of MRSA caused respiratory tract infection. This study shows that Gram positive bacteria can cause serious infections in intensive care units. The most common isolates were S. aureus followed by CONS the isolates were resistant to most of the drugs. For Gram positive cocci the most effective antibiotics were vancomycin followed by linezolid and cotrimoxazole. Appropriate antibiotic utilization in Intensive Care Units is crucial not only in ensuring an optimal outcome but also in preventing multidrug resistant bacteria.


Keywords: Nosocomial infection, Gram positive bacteria, MRSA, Antibiotic susceptibility pattern
Download this article as Download

How to cite this article:

Zarrin Afroz, Basavaraj C. Metri, Faisal Nasim Gilani and Jyothi, P. 2018. Bacteriological Profile and Antibiogram of Gram Positive Nosocomial Isolates from Intensive Care Units with Special Reference to Methicillin Resistant Staphylococcus aureus (MRSA) in a Tertiary Care Hospital of North Karnataka, India.Int.J.Curr.Microbiol.App.Sci. 7(10): 3259-3269. doi: https://doi.org/10.20546/ijcmas.2018.710.377