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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 3021-3029
DOI: https://doi.org/10.20546/ijcmas.2018.710.352


Electrophoretic Characterization of Gynoecious and Monoecious Cucumber (Cucumis sativus L.) Genotypes Based on Seed Protein Profiles
Shailaja Punetha1*, Basavaraj Makanur2, Deepali Tewari1 and Parul Punetha3
1Department of Vegetable Science, 2Department of Seed Science and Technology, 3Department of Floriculture and Landscape Architecture, G.B. Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, India
*Corresponding author
Abstract:

Thirteen (Three gynoecious and ten monoecious) germplasm lines of cucumber (Cucumber sativus L.) were characterized by sodium dodecyl sulphate polyacrylamide vertical slab gel electrophoresis (SDS-PAGE). The seed protein could be resolved into total 11 bands distributed in 4 zones i.e. A, B, C and D. Zone A was divided into 5 subzones and 5 bands, zone B has 1 band C has 5 and zone D included 5 bands. Similarity index value ranged from 62% to 100% among all the genotypes. Pgyn-1 showed least similarity 68% with other genotypes. It was observed that all the gynoecious genotypes were dissimilar to monoecious genotypes. It was possible through seed protein profiles to distinguished morphologically similar genotypes. Hence, seed protein profiles proved useful in identifying gynoecious and monoecious lines of cucumber.


Keywords: Cucumber, Protein profiling, SDS-PAGE, Germplasm, Genetic diversity, Electrophoresis
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How to cite this article:

Shailaja Punetha, Basavaraj Makanur, Deepali Tewari and Parul Punetha. 2018. Electrophoretic Characterization of Gynoecious and Monoecious Cucumber (Cucumis sativus L.) Genotypes Based on Seed Protein Profiles.Int.J.Curr.Microbiol.App.Sci. 7(10): 3021-3029. doi: https://doi.org/10.20546/ijcmas.2018.710.352