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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 2910-2918
DOI: https://doi.org/10.20546/ijcmas.2018.710.338


Isolation of Trichoderma harzianum and Evaluation of Antagonistic Potential against Alternaria alternata
K.V.M.S. Chaitanya, Harison Masih and Pakkala Abhiram*
Department of Industrial Microbiology, Jacob Institute of Biotechnology and Bioengineering, SHUATS, India
*Corresponding author
Abstract:

Chilli is one of the important spices and cultivated around the world for its peculiar hot taste. Chilli leafs prone to affect by Alternaria alternata (leaf spot). In order to overcome this problem, efforts have been made to evaluate the efficiency of the bio-control agent Trichoderma harzianum for controlling the leaf spot disease in chilli. Trichoderma harzianum was isolated from the field soil and identified through microscopic and recommended standard methods. The antagonistic activity of the Trichoderma harzianum was evaluated against the pathogenic fungi responsible for leaf spot disease. Alternaria alternata belongs to the sub-division Deuteromycotina, class Dothideomycetes, family Pleosporaceae. The mycelium of Alternari alternata is septate, brown to brownish grey in colour. The conidiophores are dark, septate, arise in fascicles, measuring 14-74 × 4-8 μm. Conidia are brownish black, obclavate, borne singly or sparingly in chains of 2-4, muriform with long beak and the overall conidial size ranges between 148-184 × 17-24 μm with 10-11 transverse and Alternaria alternata proved the efficiency to inhibit. The antagonistic activity of Trichoderma harzianum was screened in vitro against Alternaria alternata by dual culture plate technique on PDA media for 7days. The results revealed Trichoderma harzianum was shown inhibition of 60.71% over Alternaria alternata.


Keywords: Trichoderma harzianum, Alternaria alternate, Inhibition, Dual culture plate technique
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How to cite this article:

Chaitanya, K.V.M.S., Harison Masih and Pakkala Abhiram. 2018. Isolation of Trichoderma harzianum and Evaluation of Antagonistic Potential against Alternaria alternata.Int.J.Curr.Microbiol.App.Sci. 7(10): 2910-2918. doi: https://doi.org/10.20546/ijcmas.2018.710.338