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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 2891-2899
DOI: https://doi.org/10.20546/ijcmas.2018.710.336


Antioxidant and Antimicrobial Activity of Protein Hydrolysate Prepared From Tilapia Fish Waste by Enzymatic Treatment
Srikanya1, K. Dhanapal1*, K. Sravani1, K. Madhavi2, B. Yeshdas3 and Praveen Kumar1
1Department of Fish Processing Technology, 2Department of Aquatic Environment Management, 3Department of Aquaculture, College of Fishery Science, Muthukur, Nellore District, Andhra Pradesh, India
*Corresponding author
Abstract:

The antioxidant and antimicrobial properties of fish protein hydrolysate (FPH) prepared from Tilapia waste was evaluated. The Protein hydrolysates was prepared from tilapia (Oreochromis niloticus) waste using papain enzyme under the optimum conditions viz., temperature of 50°C, pH of 6.5, E/S ratio of 1% and 60 minutes time. The prepared proteinhydrolysate was evaluated for antioxidant and antimicrobial properties in vitro at different concentrations. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of FPH was highest at 100 mg/L (77.40%) and the maximum metal chelating activity of FPH was seen at 500mg/L (49.27%). The DPPH radical scavenging activity and metal chelating activity showed concentration dependent activity. FPH showed ferric reducing activity at all concentrations and exhibited highest reducing power at 500 µg /mL (2.18%). The antibacterial activity of FPH was seen at a concentration of 8 and 10 mg /mL FPH was potentially active against gram+ve bacteria viz., Staphylococcus aureus and Bacillus subtilis whereas, it showed smaller zones of inhibition against gram-ve Escherichia coli bacteria.


Keywords:

Tilapia, Papain, FPH, Antioxidant, Antimicrobial

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How to cite this article:

Srikanya, A., K. Dhanapal, K. Sravani, K. Madhavi, B. Yeshdas and Praveen Kumar, G. 2018. Antioxidant and Antimicrobial Activity of Protein Hydrolysate Prepared from Tilapia Fish Waste by Enzymatic Treatment.Int.J.Curr.Microbiol.App.Sci. 7(10): 2891-2899. doi: https://doi.org/10.20546/ijcmas.2018.710.336