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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 2807-2816
DOI: https://doi.org/10.20546/ijcmas.2018.710.326


Expression Profiling of Glycoprotein D Gene of Bovine Herpes Virus in Madindar by Bovine Kidney Cells via Real Time PCR
Asmita Singh* and Ajay Kumar
Animal Nutrition Division, ICAR- Indian Veterinary Research Institute,Bareilly, UP, 243122, India
*Corresponding author
Abstract:

Bovine herpesvirus-1 (BHV-1) a member of the Herpes viridae family and Alphaherpes viridae subfamily genus Varicello virus is a most common viral pathogen found in bovine semen. The entry process of Alphaherpes viruses consist of two steps, initial virus attachment and subsequent virus penetration involving membrane fusion as in other alphaherpes viruses, BHV-1 glycoproteins are the major structural components of the viral envelope and virus-infected cell membranes. Glycoprotein D(gD), of BHV-1 is one of four major glycoproteins, namely gB, gC, gD, and gH that have been identified on the virus envelope and the plasma membranes of BHV-1-infected cells [14, 24]. Total of 73 open reading frames (ORFs) have been clearly identified which are homologous to genes found in other alpha herpes viruses. The BHV-1 genome encloses ten genes encoding the glycoproteins, among them, six genes are in the UL region, namely gK (UL 53), gC (UL 44), gB (UL 27), gH (UL 22), gM (UL 10), gL (UL 1) and four remaining genes are in the Us region, gG (US 4), gD (US 6), gI (US 7) and gE (US 8). In the present study, a time course expression profile analysis of gD (US 6), was carried out at at 6hr, 12hr, 24hr of h post infection quantitative RT-PCR RT-PCR as well as Real Time PCR. Gapdh was used as reference control to normalize the expression levels. A significant decrease in gD expression at 3 h post infection (h.p.i) was observed followed by an increase in between 6 hr to 12 hr h.p.i. Expression of gC gene become static in between 12 to 24 hr. However, further studies need to be carried out to unfold the molecular mechanism of interaction between host and virus to devise efficient control strategies for this chaos in the virus infection.


Keywords: Glycoprotein D, Expression, Alphaherpes viruses, Herpes viridae
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How to cite this article:

Asmita Singh and Ajay Kumar. 2018. Expression Profiling of Glycoprotein D Gene of Bovine Herpes Virus in Madindar by Bovine Kidney Cells via Real Time PCR.Int.J.Curr.Microbiol.App.Sci. 7(10): 2807-2816. doi: https://doi.org/10.20546/ijcmas.2018.710.326