Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 2799-2806
DOI: https://doi.org/10.20546/ijcmas.2018.710.325


Molecular Characterization of Trichoderma Mutants with RAPD Marker
Radhika Deshmukh, P.N. Davhale and Maya Nadare
Department of Plant Pathology, Post Graduate Institute, Dr. PDKV Akola (MS) 444104, India
*Corresponding author
Abstract:

Trichoderma is an important biocontrol agent of several soil borne plant pathogens. The antagonistic ability of a biocontrol agent was determined by its physiological state so that change in physiological or genetical condition could alter the antagonism. Genetic modification using mutagenesis offers the potential for producing improved bio protection is likely to enhance their biocontrol capabilities against soil borne pathogen. Molecular techniques are important analytical tool to characterized genetic variability and diagnosis of microbial population. Molecular variability of Trichoderma mother culture and its mutants was studied by using Random Amplified Polymorphic DNA (RAPD). 20 RAPD primer of OPA series were tested, of which 14 primers produced 72 scorable bands among them 52 bands were polymorphic and level of polymorphism was upto 72%. On the basis of dendrogram, the tested cultures were clearly divided into 2 groups. First group i.e. cluster A included 4 mutants TvM-5, TvM-3, TvM-1 TvM-2 and mother culture TvMC. Second group i.e. cluster B included only one mutant i.e. TvPM-4.


Keywords: Trichoderma mutants, DNA extraction, RAPD marker, Scorable bands
Download this article as Download

How to cite this article:

Radhika Deshmukh, P.N. Davhale and Maya Nadare. 2018. Molecular Characterization of Trichoderma Mutants with RAPD Marker.Int.J.Curr.Microbiol.App.Sci. 7(10): 2799-2806. doi: https://doi.org/10.20546/ijcmas.2018.710.325