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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 2438-2445
DOI: https://doi.org/10.20546/ijcmas.2018.710.282


Elevation of Nitric Oxide Level in Rohu (Labeo rohita) in Response to Immunization with Whole Antigens of Fish Ectoparasite, Argulus siamensis
P. Das1, 2, J. Mohanty1*, M.R. Badhe1, P.K. Sahoo1, K.K. Sardar2 and S.C. Parija2
1ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar-751002, India
2Department of Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Bhubaneswar-751003, India
*Corresponding author
Abstract:

Argulosis, caused predominantly by Argulus siamensis is a threatening ectoparasitic disease of Indian carp aquaculture. Vaccination against this parasite is a safe alternative to the harmful chemicals used for its control. Nitric oxide (NO), a signaling molecule plays an important role in immune mediated functions in different parasitic diseases. NO mediates immune response through cytokine production and gives protection against parasitic diseases by vaccination or immunization. In the present study, the level of NO production in response to Argulus siamensis whole antigen immunization in rohu (Labeo rohita) was assessed by Griess method in serum and two tissue samples (kidney and liver). There was significant increase in NO in serum (39.27 vs 15.57 nmol/ml), kidney (0.66 vs 0.17 nmol/mg tissue) and liver (0.61 vs 0.16 nmol/mg tissue) in immunized fish compared to the control fish. Further the immunized fish were confirmed for the presence of antibody against the Argulus parasite by dot blot method. The results possibly confirm the increased level NO possessing protective or immune-related function against this parasitic disease.


Keywords: Nitric oxide, Labeo rohita, Argulus siamensis, Immune response
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How to cite this article:

Das, P., J. Mohanty, M.R. Badhe, P.K. Sahoo, K.K. Sardar and Parija, S.C. 2018. Elevation of Nitric Oxide Level in Rohu (Labeo rohita) in Response to Immunization with Whole Antigens of Fish Ectoparasite, Argulus siamensis.Int.J.Curr.Microbiol.App.Sci. 7(10): 2438-2445. doi: https://doi.org/10.20546/ijcmas.2018.710.282