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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 1662-1669
DOI: https://doi.org/10.20546/ijcmas.2018.710.190


Studies on Induced Chemical Mutagenesis in Groundnut (Arachis hypogaea L.)
Mayur V. Jadhav, Rahul K. Zote and Amol D. Sable
Department of Plant Biotechnology SDMVM’s College of Agricultural Biotechnology, Georai Tanda, Paithan Road, Aurangabad (M.S.) - 431001, India
*Corresponding author
Abstract:

Present investigation entitled “Studies on induced chemical mutagenesis in groundnut (Arachis hypogaea L.) was carried out during January 2018 – March 2018 in Department of Plant Biotechnology, SDMVM’S College of Agriculture Biotechnology, Georai Tanda, Aurangabad. Experiment was laid out in Randomly Block Design with two mutagens six treatment and three replications. The groundnut variety (LGN-1) from oilseed research station, Latur was considered as base population for present mutation breeding experiments. This study was performed by exposing the seeds of groundnut (Arachis hypogaea L.) to ethyl methane sulphonate [EMS] sodium azide [SA] to study their effects on various morphological traits when compared with control. The seeds of groundnut were pre-soaked in distilled water for 6 hours for ems and 24 hrs for SA. The SA, EMS solution was prepared in distilled water. Each concentration of SA, EMS was prepared in 100ml of distilled water for 300 groundnut seeds. These soaked seeds treated with different concentration of SA, EMS such as 0.2%, 0.4%, 0.6%, 0.8, 0.10% and 0.12 %. After treatment, carefully remove the SA, EMS solution and treated seeds were washed under running tap water to remove the traces of mutagen sticking to the seed coat The observations were made on seed germination, plant height, number of branches, number of leaves per plant and plant survival (%) after treating seeds with different mutagens. Present investigation showed significant results in seed germination, plant height, number of branches and number of leaves per plant and plant survival (%) after treating seeds with different mutagens.


Keywords: Groundnut, Sodium azide, EMS, Mutagenesis
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How to cite this article:

Mayur V. Jadhav, Rahul K. Zote and Amol D. Sable. 2018. Studies on Induced Chemical Mutagenesis in Groundnut (Arachis hypogaea L.).Int.J.Curr.Microbiol.App.Sci. 7(10): 1662-1669. doi: https://doi.org/10.20546/ijcmas.2018.710.190