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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 1348-1356
DOI: https://doi.org/10.20546/ijcmas.2018.710.150


Isolation and Molecular Characterization of Brucella melitensis from Abortion Storms in Sheep with its Zoonotic Implications
D.K. Sharma1, B. Bhardwaj2, H.C. Chouhan3, B. Joseph1 and S.K. Barolia4*
1Department of Microbiology, College of Veterinary and Animal Sciences, Navania, Vallabhanagar, Udaipur, Rajasthan – 313601, India
2Regional Disease Diagnostic Center, Department of Animal Husbandry Udaipur, Rajasthan, India
3Department of Microbiology & Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar, Dantiwada, Gujarat-385506, India
4Department of Zoology, University College of Science, ML Sukhadia University, Udaipur, Rajasthan-313001, India
*Corresponding author
Abstract:

Outbreak of brucellosis has been recorded in sheep flocks in Udaipur district of Rajasthan, India. Total 15 abortions were recorded in two flocks of 115 sheep. Samples from aborted fetus, retained placenta colostrum and sera samples were collected. Sera samples were tested by Rose Bengal plate agglutination test. Culture and isolation were done on selective media. All tested sera samples were found positive for brucellosis while Brucella melitensis was isolated from fetal abomasal content and placenta samples. Three isolated strains were subjected to molecular characterization by Real time PCR using BCSP31 gene specific oligonucliotide primer pair in real time PCR with SYBR Green chemistry. Further these isolates were confirmed by Taqman probe chemistry with IS711 gene. Species level characterization was done by Bruce ladder PCR. Outbreak of Brucellosis has been confirmed. Three strains were isolated and by molecular characterization found to be Brucella melitensis. All sheep examined found serologically positive for brucellosis. This is a primary report of culture confirmation of Brucella melitensis outbreak in southern Rajasthan. Study show the occurrence of Brucella melitensis in the area which is of major zoonotic concern. Here we are reporting first time isolation and molecular confirmation of Brucella melitensis in sheep from Udaipur district of Rajasthan


Keywords: Brucellosis, Real time PCR, Brucella melitensis, Sheep, Udaipur, Rajasthan
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How to cite this article:

Sharma, D.K., B. Bhardwaj, H.C. Chouhan, B. Joseph and Barolia, S.K. 2018. Isolation and Molecular Characterization of Brucella melitensis from Abortion Storms in Sheep with its Zoonotic Implications.Int.J.Curr.Microbiol.App.Sci. 7(10): 1348-1356. doi: https://doi.org/10.20546/ijcmas.2018.710.150