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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 1280-1291
DOI: https://doi.org/10.20546/ijcmas.2018.710.144


Green Synthesis of Zinc Nanoparticles using Pseudomonas fluorescens Extract and Their Antibacterial Activity against Xanthomonas spp.
J.U. Vinay1*, V.B. Nargund1, S. Jahagirdhar1, R.R. Patil2 and R.V. Hegde3
1Department of Plant Pathology
2Department of Agricultural Entomology
3Department of Horticulture, College of Agriculture, University of Agricultural Sciences, Dharwad - 580 005, Karnataka, India
*Corresponding author
Abstract:

In the field of plant pathology, exploitation of metal nanoparticles in bacterial disease management is a novel and needy approach. Green synthesis of nanoparticles is most prioritized because of their safe, eco-friendly nature and cost effectiveness. A total of 16 fluorescent pseudomonad isolates were obtained from different crop rhizosphere in various locations. The isolates were screened for zinc utilization capabilities. Among the 16 isolates, one isolate (FPGrChHi-6) showed maximum zone of zinc utilization (30.00 mm) with bacterial growth (12.00 mm). Molecular characterization of isolate revealed that, isolate FPGrChHi-6 belongs to Pseudomonas fluorescens. Further, zinc nanoparticles (ZnNPs) were synthesized by using P. fluorescens extract. The green synthesized ZnNPs were characterized using various instruments. The UV-vis spectrophotometer analysis showed the prominent peak at 360 nm, which indicated the synthesis of ZnNPs. The characterization through particle size analyzer and atomic force microscope found that, the mean diameter of ZnNPs was found to be 21.40 nm with spherical to irregular in shape. ZnNPs showed phytotoxicity symptoms on tomato seedlings at 1500 ppm concentration and onwards. ZnNPs showed excellent antibacterial activity against Xanthomonas oryzae pv. oryzae and Xanthomonas axonopodis pv. punicae. Among tested concentrations, maximum inhibition zones were noticed against Xap (21.76 mm) and Xoo (15.67 mm) at 1000 ppm and 1250 ppm concentrations respectively.


Keywords: Zinc utilization, Zinc nanoparticles, Pseudomonas fluorescens, Antibacterial activity, Xanthomonas spp.
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How to cite this article:

Vinay, J.U., V.B. Nargund, S. Jahagirdhar, R.R. Patil and Hegde, R.V. 2018. Green Synthesis of Zinc Nanoparticles using Pseudomonas fluorescens Extract and Their Antibacterial Activity against Xanthomonas spp.Int.J.Curr.Microbiol.App.Sci. 7(10): 1280-1291. doi: https://doi.org/10.20546/ijcmas.2018.710.144