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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 1160-1165
DOI: https://doi.org/10.20546/ijcmas.2018.710.129


Prevalence and Detection of Cytomegalovirus by Polymerase Chain Reaction (PCR) in Pregnant Women
C. Revathy1, G. Velvizhi1 and G. Sucilathangam2*
1Department of Microbiology, Tirunelveli Medical College, Tirunelveli - 627 011, Tamil Nadu, India
2Department of Microbiology, Government Theni Medical College, Theni - 625 512, Tamil Nadu, India
*Corresponding author
Abstract:

Cytomegalovirus infections are endemic worldwide. The most frequently used methods for detecting antibodies in developing world are the Enzyme Linked Immunosorbent Assay. The polymerase chain reaction is a molecular biology technique in which the production of large amounts of specific deoxyribonucleic acid fragments is induced from very low concentrations of complex substrates allowing the detection of very low amounts of viral particles. Blood samples were collected from pregnant women with bad obstetric history attending Outpatient Department and were stored at -20°C and transported to the main Laboratory of Department of Microbiology where DNA was extracted from the serum and PCR was performed for CMV DNA amplification. A total of 47 women were screened, amongst them, 4 (8.5%) were cytomegalovirus (CMV) DNA positive by polymerase chain reaction (PCR) whereas 47 Blood donors were screened, amongst them, 1 (2.1%) were cytomegalovirus (CMV) DNA positive. Prevalence was recorded in age group 21 to 30 years. The detection of HCMV in women before pregnant should reduce miscarriage rate and also the number of congenitally infected infant. Real-time PCR is the best technique and more sensitive and effect than conventional PCR and ELISA.


Keywords: Cytomegalovirus, Enzyme Linked Immunosorbent Assay, Polymerase Chain Reaction, CMV DNA
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How to cite this article:

Revathy, C., G. Velvizhi and Sucilathangam, G. 2018. Prevalence and Detection of Cytomegalovirus by Polymerase Chain Reaction (PCR) in Pregnant Women.Int.J.Curr.Microbiol.App.Sci. 7(10): 1160-1165. doi: https://doi.org/10.20546/ijcmas.2018.710.129