Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 901-906
DOI: https://doi.org/10.20546/ijcmas.2018.710.100


Scanning Electron Microscopy (SEM) Study Showed the Mode Action of Metarhizium anisopliae on Odontotermes obesus
Pranab Dutta, J. Pegu, H. Kaushik, P. Kaman and Ankita Das
Department of Plant Pathology, Assam Agricultural University, Jorhat-785013, Assam, India
*Corresponding author
Abstract:

The present study was done to investigate the histopathological effect of the entomopathogenic fungi, Metarhizium anisopliae on Odontotermes obesus using Scanning Electron Microscopy (SEM). Adult worker termites were inoculated with 1x109 conidia/ml suspension of the fungi and maintained at 25±1°C temperature and 80 % relative humidity. SEM study showed that when this mitotic (asexual) spore (called conidia) of the fungus came into contact with the body of the termite, they germinated and the hyphae of the fungus that emerged penetrated the cuticle. The hyphal penetration of the fungi through the insect cuticle as well as proliferation formed a dense network of mycelium in the insect cuticle. This fungus also invaded the host through the integument and caused death of the insect. This lethal effect to the insect is very likely to be aided by the production of insecticidal cyclic peptides (destruxins) by the fungus. SEM study revealed that it is a convenient tool to observe the mode of action of entomopathogenic fungi and to observe how they are able to colonize and infect the host.


Keywords: Metarhizium anisopliae, Odontotermes obesus, Scanning Electron Microscopy
Download this article as Download

How to cite this article:

Pranab Dutta, J. Pegu, H. Kaushik, P. Kaman and Ankita Das. 2018. Scanning Electron Microscopy (SEM) Study Showed the Mode Action of Metarhizium anisopliae on Odontotermes obesus.Int.J.Curr.Microbiol.App.Sci. 7(10): 901-906. doi: https://doi.org/10.20546/ijcmas.2018.710.100