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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 663-679
DOI: https://doi.org/10.20546/ijcmas.2018.710.074


Comparison of the Immunogenicity of an Inactivated Classical Swine Fever Virus Vaccine Employing a Local Isolate With the Live Lapinized Classical Swine Fever Virus Vaccine
K.H. Steffi Rajakumar1, A. Ramesh2, K.G. Tirumurugaan2 and K. Kumanan3
1Department of Veterinary Microbiology, MVC, Chennai-7, Tamil Nadu, India
2Vaccine Research Centre- Viral Vaccines, Centre for Animal Health Studies, TANUVAS, Chennai-51, Tamil Nadu, India
3Department of Animal Biotechnology, MVC, TANUVAS, Chennai-7; 4- MVC, TANUVAS, Chennai-51, Tamil Nadu, India
*Corresponding author
Abstract:

A study was conducted for assessing the immunogenicity of the inactivated PK-15 cell adapted classical swine fever virus (CSFV) vaccine in pigs under farm conditions. Thirty numbers of pigs of 10 -12 weeks of age maintained at two farms each were vaccinated. The 30 number of pigs from each farms were divided into three groups comprising of 10 animals each. Group I was vaccinated with the inactivated PK-15 cell adapted CSF virus vaccine adjuvanated with single (Oil-in-water O/W) emulsion. Group II was vaccinated with the inactivated PK-15 cell adapted CSF virus vaccine adjuvanated with double (water-in-oil-in-water W/O/W) emulsion. Group III was vaccinated with the lapinized live CSFV vaccine. In addition, Group IV and V comprising 6 pigs each in farm located at Madhavaram milk colony, Chennai 51 were vaccinated with a booster dose with two types of inactivated PK-15 cell adapted CSFV vaccine with single and double emulsion adjuvant respectively on 28th day after primary vaccination. With regard to humoral immune responses, the inactivated CSFV vaccine emulsified with single emulsion adjuvant generated mean humoral immune response lower than the threshold serum antibody levels (PI of 50%) in the ELISA assay at 28 dpv and comparable immune responses on 90 and 180 dpvwere observed in comparison with the lapinized live CSFV vaccine. However, the single emulsion vaccine was found effective in inducing humoral immune response in the absence of maternal antibodies. Farm trials of the inactivated CSFV vaccine with single emulsion adjuvant could generate and sustain the serum CSFV antibody levels until 180 dpv which is a major requirement under field conditions since the pigs are marketed at 8-9 months of age.


Keywords: Inactivated adjuvanted CSF vaccine, ELISA, Immunogenicity
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How to cite this article:

Steffi Rajakumar, K.H., A. Ramesh, K.G. Tirumurugaan and Kumanan, K. 2018. Comparison of the Immunogenicity of an Inactivated Classical Swine Fever Virus Vaccine Employing a Local Isolate With the Live Lapinized Classical Swine Fever Virus Vaccine.Int.J.Curr.Microbiol.App.Sci. 7(10): 663-679. doi: https://doi.org/10.20546/ijcmas.2018.710.074