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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 520-528
DOI: https://doi.org/10.20546/ijcmas.2018.710.057


Comparative Study of Different Tests for Diagnosis of Sub Clinical Mastitis in Buffaloes
Rakesh Kumar1*, Yudhbir Singh Rana2 and Anshu Sharma3
1Department of Animal Husbandry & Dairying, Haryana, India
2Department of Veterinary Medicine, LUVAS, Hisar, Haryana, India
3Incharge College Central Laboratory, COVSc, LUVAS, Hisar, Haryana, India
*Corresponding author
Abstract:

In the present investigation, 235 quarter milk samples from 59 apparently healthy lactating buffaloes were collected to compare different diagnostic tests for detection of Sub-Clinical Mastitis (SCM) in Buffaloes. Out of 235 quarter milk samples, 17.02 per cent, 16.59 per cent, 16.17 per cent, 14.49 per cent, 14.46 per cent and 17.02 per cent quarters were tested positive for SCM by bromothymol blue (BTB) strip test, electrical conductivity (EC), California mastitis test (CMT), N-acetyl-β-D-glucosaminidase (NAG-ase), Somatic cell count (SCC) and Cultural Examination, respectively. While calculating percent sensitivity, specificity and predictive value of these tests taking cultural examination as standard, the NAG-ase test was found to have highest sensitivity (60%), Specificity (94.87%) and predictive value (70.58%) followed by SCC, CMT, EC and BTB strip test, respectively. NAG-ase test can be used as a preferential laboratory tool over SCC and cultural examination, however CMT can be used as a first screening tool for detecting suspected samples for further investigation for SCM.


Keywords: Buffaloes, Electrical conductivity, Mastitis, NAG-ase test, Somatic cell count
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How to cite this article:

Rakesh Kumar, Yudhbir Singh Rana and Anshu Sharma. 2018. Comparative Study of Different Tests for Diagnosis of Sub Clinical Mastitis in Buffaloes.Int.J.Curr.Microbiol.App.Sci. 7(10): 520-528. doi: https://doi.org/10.20546/ijcmas.2018.710.057