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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 449-454
DOI: https://doi.org/10.20546/ijcmas.2018.710.048


Prevalence and Molecular Diagnosis of Escherichia coli in captive Sloth Bears (Melursus ursinus)
M. Palanivelrajan1*, A. Sha Arun2, M.G. Jayathangaraj3, K. Vijayarani4, Bhaskaran Ravi Latha5, P. Sridevi6 and K. Porteen7
1Department of Wildlife Science
3Department of Veterinary Clinical Medicine
4Department of Animal Biotechnology
5Department of Veterinary Parasitology
6Department of Clinics
7Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu-600 007 India
2Research & Veterinary Operations, Wildlife SOS, Bannerghatta Bear Rescue Centre, Bannerghatta Biological Park, Bangalore – 560 083 Karnataka, India
*Corresponding author
Abstract:

Pathogenic Escherichia coli strain from faeces of sloth bears was screened by using culture morphology, Gram’s staining, biochemical tests and polymerase chain reaction. Our results showed that out of 60 samples collected, 59 samples (98.33%) were cultured on EMB agar and Gram’s stain revealed rod-shaped Gram-negative organism with green metallic sheen - like colonies. The biochemical tests of cultured samples revealed positive to indole production, positive to methyl red test, negative to Voges-Proskauer test, negative to Simmon citrate utilization test, positive to glucose, negative to H2S production and negative to urea production. All E. coli isolates were found sensitive to azithromycin, streptomycin and tetracycline. They were found intermediate to enrofloxacin and gentamicin. The fimC gene was amplified by PCR for the pathogenic Escherichia coli and were found to be positive of 53.33% among the juveniles, 46.67% among the sub-adults, 53.33% among the adults and 33.33% among the geriatrics were found to be positive. This study may provide information for developing strategies in the future in the control of Escherichia coli infections in sloth bears.


Keywords: Antibiogram, Escherichia coli, fimC gene, Sloth Bear, Ursidae
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How to cite this article:

Palanivelrajan, M., A. Sha Arun, M. G. Jayathangaraj, K. Vijayarani, Bhaskaran Ravi Latha, P. Sridevi and Porteen, K. 2018. Prevalence and Molecular Diagnosis of Escherichia coli in captive Sloth Bears (Melursus ursinus).Int.J.Curr.Microbiol.App.Sci. 7(10): 449-454. doi: https://doi.org/10.20546/ijcmas.2018.710.048