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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 318-323
DOI: https://doi.org/10.20546/ijcmas.2018.710.033


Prevalence of Theileria equi among Horses Based on Parasitological and Universal Molecular Technique
T.M. Vidhyalakshmi1*, Sunant K. Raval1, Bharat B. Bhanderi2 and Amit K. Kanani3
1Department of Veterinary Medicine
2Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
3Animal Disease Investigation Office, Ahmedabad, Gujarat, India
*Corresponding author
Abstract:

Equine theileriosis, being an OIE listed disease mainly requires frequent disease screening and monitoring. The aim of the present study was to monitor the prevalence of theileriosis in horses by using both parasitological method and molecular diagnostic technique based on Equine Merozoite Antigen-5 and 6. In the present study, samples from 248 horses were collected randomly. Positive samples detected by using Giemsa stained blood smear technique (GSBT) were used as the positive control samples for screening randomly collected blood samples by using Polymerase chain reaction technique (PCR) based on Equine Merozoite Antigen-5 and 6. Out of 248 horse samples screened for Theileria equi DNA, 30 were positive indicating an overall prevalence rate of 12.10% by universal PCR whereas GSBT revealed only 5.05% prevalence. This study emphasized the usefulness of universal PCR technique as an efficient diagnostic tool for determination of T. equi in field samples rather than Giemsa stained blood smear technique.


Keywords: Blood samples, Equine merozoite antigen, Polymerase chain reaction, Prevalence, Theileria equi
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How to cite this article:

Vidhyalakshmi, T.M., Sunant K. Raval, Bharat B. Bhanderi and Amit K. Kanani. 2018. Prevalence of Theileria equi among Horses Based on Parasitological and Universal Molecular Technique.Int.J.Curr.Microbiol.App.Sci. 7(10): 318-323. doi: https://doi.org/10.20546/ijcmas.2018.710.033