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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 257-262
DOI: https://doi.org/10.20546/ijcmas.2018.710.027


Genetic Diversity Analysis in Chilli (Capsicum annuum L.) Found in Manipur Using RAPD Markers
Atom Annupama Devi1*, Naorem Brajendra2 and Mutum Dinachandra3
1Department of Plant Molecular Genetics and Genomics, Institute of Bioresources and Sustainable Development, Takyel, Manipur, India
2Department of Genetics and Plant Breeding, Central Agricultural University, CAU, Imphal, India
3Department of Horticulture, Central Agricultural University, CAU, Imphal, India
*Corresponding author
Abstract:

The present study was undertaken to understand the genetic diversity of Chilli found in Manipur using RAPD primers. Twenty (20) primers were screened, out of which 9 primers gave polymorphic bands which were used for further analysis. The polymorphic information content (PIC) values ranged from 0.2 (OPB-15) to 0.45 (OPW-04) with an average of 0.35. The resolving power was found highest in OPW04 with value of 7.44 and lowest in OPA11 (2.10). Overall, it was found that OPW04 is a good primer which produced 100% polymorphism with high discriminating power. Cluster analysis using Un-weighted Pair Group Method with Arithmetic Averages (UPGMA) grouped all the chilli samples into three major clusters at a distance coefficient of 0.50, major cluster 1 consist of 4 cultivars (CAU 1, CAU 4, CAU 9, CAU 10). The 2nd major clusters consist of 6 cultivars- (CAU 2, CAU 5, CAU 7, CAU 6, CAU 8, CAU 3. The 3rd major cluster consist of only 1 cultivar (CAU 11).The results showed that RAPD can be effectively used to analysed genetic diversity of chilli found in Manipur.


Keywords: Genetic diversity, Manipur, polymorphic, RAPD, Polymorphic information content
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How to cite this article:

Atom Annupama Devi, Naorem Brajendra and Mutum Dinachandra. 2018. Genetic Diversity Analysis in Chilli (Capsicum annuum L.) Found in Manipur Using RAPD Markers.Int.J.Curr.Microbiol.App.Sci. 7(10): 257-262. doi: https://doi.org/10.20546/ijcmas.2018.710.027