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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 234-240
DOI: https://doi.org/10.20546/ijcmas.2018.710.024


Identification and Antimicrobial Susceptibility testing of Non Fermenting Gram Negative Bacteria by Vitek 2 in a Teaching Hospital in Mahbubnagar
Srividya Yeruva* and Vasantha Kabra
Department of Microbiology, SVS Medical College, Yenugonda, Mahbubnagar, Telangana, 509001, India
*Corresponding author
Abstract:

Non fermenting gram negative bacilli (NFGNB) are aerobic, non-spore forming bacteria. Although previously considered as contaminants, their pathogenic potential was proved by their frequent isolation from clinical samples and association with diseases. Aim: To identify NFGNB from various clinical samples and perform antimicrobial susceptibility testing (AST) by Vitek 2. Materials and methods: All the clinical samples received for routine bacterial culture in Department of Microbiology, SVS Medical College, from January 2017 to April 2018 were plated on agar plates and non-lactose fermenting colonies on MacConkey agar plates were further processed by Vitek 2. Results: A total of 126 NFGNB were isolated from various clinical samples and their AST was evaluated by Vitek 2. Pseudomonas aeruginosa (66.66%) and Acinetobacter baumanii (23.80%) were most common NFGNB isolated. High variability in antibiotic resistance pattern was noted in the present study. 47.61 % of NFGNB exhibited Multidrug resistance (MDR) and 3.37% Pseudomonas aeruginosa strains showed Pandrug resistance (PDR). Conclusion: Accurate and rapid identification and antimicrobial susceptibility testing of NFGNB by Vitek 2, help in early initiation of appropriate antimicrobial therapy and proper management of patients and will reduce emergence of MDR and PDR strains of NFGNB.


Keywords: NFGNB, Pseudomonas aeruginosa, Acinetobacter baumanii, Stenotrophomonas maltophilia, Burkholderia cepacia, Vitek 2, MDR, PDR
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How to cite this article:

Srividya Yeruva and Vasantha Kabra. 2018. Identification and Antimicrobial Susceptibility testing of Non Fermenting Gram Negative Bacteria by Vitek 2 in a Teaching Hospital in Mahbubnagar.Int.J.Curr.Microbiol.App.Sci. 7(10): 234-240. doi: https://doi.org/10.20546/ijcmas.2018.710.024