National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The purpose of this work is to assess microbial contamination level of millet and sorghum in Benin and to identify the molds responsible for this contamination. Sampling (240 millet and 240 sorghum grain) was done in Beninese agro-ecological zones (I to V) where both cereals are produced. Fungi were isolated using Direct Plating methodand characterised according to macroscopic and microscopic criteria. Grains microbial density was evaluated using the suspension-dilution method. The moisture content obtained per agro-ecological zone varies from 9.12% to 10.42% for millet and from 10.71% to 12.18% for sorghum. Contamination rates range from 37.89% to 52.71% and from 41.89% to 55.11% respectively for millet samples from attics and markets. For sorghum, contamination rates vary from 42.39% to 61.36% for attics samples and from 43.46% to 74.47% for markets samples. The highest total flora was obtained in zone III (40 103 CFU/g) for millet and in zone V (113.25103 CFU/g) for sorghum. With regard to fungal load, the highest values were obtained in zones II and IV (2.5 103 CFU/g) for millet and in zone IV (14.64 103 CFU/g) for sorghum. The 535 molds strains identified mainly belong to the genera Aspergillus (52%) and Penicillium (27%) followed by Fusarium (10%) and Mucor (9%).
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