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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 3393-3399
DOI: https://doi.org/10.20546/ijcmas.2018.709.421


Mutagenic Effects of Gamma-rays and EMS on M1 Population of Rice Bean (Vigna umbellata Thunb, Ohwi & Ohashi)
Dayanidhi Mishra1, Lopamudra Bhoi2, Swapan K. Tripathy3, Mohima Prasad Behera1 and Tapas K. Mishra2
1All India Co-ordinated Research Network on Potential Crops, OUAT, Bubaneswar, India
2Department of Plant Breeding and Genetics
3Department of Agricultural Biotechnology, CA, OUAT, Bhubaneswar, India
*Corresponding author
Abstract:

Genetically pure seeds of rice bean (Vigna umbellata Thunb., Ohwi & Ohashi) variety viz., RBL 50 was used for induction of mutation by using three concentrations (0.2%, 0.4% and 0.6%) of Ethyl Methanesulphonate (EMS) and three doses of gamma rays (150Gy, 200Gy and 250Gy) to study the effect of mutagens on the M1 population of the crop. Observations on thirteen characters were recorded and subjected to appropriate statistical analysis based on the sample mean. Numerical Taxonomy (Sneath and Sokal, 1973) method was followed to classify the mean values of all the quantitative data for all M1 traits to find out the similarities among different mutagenic treatments. Invariable reduction in means of germination (%), initial plant population, plant height, length of pod and number of seeds/pod and a reverse trend for days to 50% flowering, days to maturity, branches/plant, pods/cluster, pods/plant, 100-seed weight and seed yield per plant was noticed in both the mutagenic treated populations compared to the mean of the corresponding character in control. Dose dependent reduction in means of characters for germination (%), initial plant population, plant height and seed yield per plant was observed in all the mutagenic treatments. Similar trend was also observed for clusters/plant, branches/plant, length of pod in gamma rays treatments and pods per plant and seeds per pod in EMS treated populations. Mutagenic treatments with below LD50 dose of mutagens were grouped in Cluster-I (0.2% EMS, 150Gy and 200Gy), EMS (0.6%) in Cluster-II, medium dose mutagenic treatments (0.4% EMS and 250Gy) in Cluster-III and control in Cluster-IV. Grouping of mutagenic treatments of different concentrations in different clusters indicated the phenological differences among the treatments.


Keywords: Gamma rays, EMS, Mutagenic effects, Rice bean
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How to cite this article:

Dayanidhi Mishra, Lopamudra Bhoi, Swapan K. Tripathy, Mohima Prasad Behera and Tapas K. Mishra. 2018. Mutagenic Effects of Gamma-rays and EMS on M1 Population of Rice Bean (Vigna umbellata Thunb, Ohwi & Ohashi).Int.J.Curr.Microbiol.App.Sci. 7(9): 3393-3399. doi: https://doi.org/10.20546/ijcmas.2018.709.421