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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 3329-3338
DOI: https://doi.org/10.20546/ijcmas.2018.709.413


Virulence Genes and Antibiotic Resistance Profile of Escherichia coli Strains Isolated From Tuna Loins and Flakes Produced in Côte D’ivoire
Andrée Emmanuelle Sika1, 3*, Ollo Kambire2, Zamblé Bi Irié Abel Boli1, Yolande Aké-Assi3 and Rose Koffi-Nevry1
1Laboratory of Biotechnology and Food Microbiology, (UFR/STA), University of Nangui Abrogoua, 02BP801 Abidjan, Côted’Ivoire
2Department of Biochemistry and Food Sciences, University of Peleforo Gon Coulibaly, BP1328, Korhogo, Côted’Ivoire
3Central Laboratory for Food Hygiene and Agribusiness, LANADA, Abidjan, Côte d’Ivoire
*Corresponding author
Abstract:

This study was conducted to characterize virulence genes of Escherichia coli isolates from tuna loins and flakes produced in Côte d’Ivoire. In total, 460 Escherichia coli isolates were analyzed for the presence of diarrhea-associated genes (eae, stx, AggR, elt, and est) by multiplex PCR using specific primers and for their susceptibility to 10 different antimicrobials according to the disk diffusion susceptibility test. 44 isolates (21 from tuna loins and 23 from tuna flakes) were positive for virulence genes, including 22 positive for elt (ETEC), 8 positive for est (ETEC) and 14 positive for both elt and est. The resistance to amoxicillin was most prevalent (15.9%), followed by that to amoxicillin/clavulanic acid (9.1%), gentamicin (2.3%) and chloramphenicol (2.3%). 4.3% were resistant to amoxicillin and gentamicin; these were consequently defined as multidrug resistant. This study revealed the presence of diarrhea genic and multidrug resistant E.coli and potential public health risks if tuna products are not appropriately cooked.


Keywords: Tuna loins, Tuna flakes, Escherichia coli, Virulence, Antibiotic resistance, Côte d’Ivoire
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How to cite this article:

Andrée Emmanuelle Sika, Ollo Kambire, Zamblé Bi Irié Abel Boli, Yolande Aké-Assi and Rose Koffi-Nevry. 2018. Virulence Genes and Antibiotic Resistance Profile of Escherichia coli Strains Isolated From Tuna Loins and Flakes Produced in Côte D’ivoire.Int.J.Curr.Microbiol.App.Sci. 7(9): 3329-3338. doi: https://doi.org/10.20546/ijcmas.2018.709.413