National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study was conducted to characterize virulence genes of Escherichia coli isolates from tuna loins and flakes produced in Côte d’Ivoire. In total, 460 Escherichia coli isolates were analyzed for the presence of diarrhea-associated genes (eae, stx, AggR, elt, and est) by multiplex PCR using specific primers and for their susceptibility to 10 different antimicrobials according to the disk diffusion susceptibility test. 44 isolates (21 from tuna loins and 23 from tuna flakes) were positive for virulence genes, including 22 positive for elt (ETEC), 8 positive for est (ETEC) and 14 positive for both elt and est. The resistance to amoxicillin was most prevalent (15.9%), followed by that to amoxicillin/clavulanic acid (9.1%), gentamicin (2.3%) and chloramphenicol (2.3%). 4.3% were resistant to amoxicillin and gentamicin; these were consequently defined as multidrug resistant. This study revealed the presence of diarrhea genic and multidrug resistant E.coli and potential public health risks if tuna products are not appropriately cooked.
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