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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 3265-3273
DOI: https://doi.org/10.20546/ijcmas.2018.709.405


Comparison of Phenotypic Methods for the Detection of Biofilm Production in Indwelling Medical Devices Used in NICU & PICU in a Tertiary Care Hospital in Hyderabad, India
Shumaila Abdul Rehman1, V.V. Shailaja1* and Appa Rao2
1Department Microbiology, Gandhi Medical College, Hyderabad, T.S., India 2Department of Microbiology, Andhra Medical College, Andhra Pradesh, India
*Corresponding author
Abstract:

Biofilms are surface associated bacterial communities surrounded by a matrix of exopolymers. Biofilms contribute to the development of persistent infections due to their refractiveness to antibiotic therapy. The purpose of this study was to evaluate three methods for detection of biofilm formation in different indwelling devices. For detection of biofilm formation, out of the 100 indwelling devices processed, 52 bacterial isolates showed growth and these were subjected for biofilm production detection by tissue culture plate (TCP) method, Tube method (TM) and Congo red agar (CRA) method. Out of the 52 isolates subjected for biofilm Production, T.C.P. Method detected 42 (80.7%), T.M. Method detected 38 (66%), C.R.A. Method detected 8 (14%) of the biofilm Producers. Out of the 42 isolates which produced biofilms by T.C.P. method, 10 (23%) were strong, 26 (62%) were moderate and 6 (14%) were weak biofilm producers. Out of the 38 isolates which produced biofilms by T.M., 4(10.5%) were strong, 28 (66%) were moderate, 8 (19%) were weak biofilm Producers. Among the different microorganisms, the frequency of biofilm production showed by 42% of P. aeruginosa isolates, 37.5% of A. baumanii, 25% E. coli, 14% of K. pneumoniae. In our study we found that TCPM was the gold standard phenotypic method used for detection of biofilms in resource limited settings. Our study did not recommend C.R.A. method for screening of biofilms due poor sensitivity


Keywords: Medical devices, Biofilm formation, Congo red, Tube method, Tissue culture plate
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How to cite this article:

Shumaila Abdul Rehman, V.V. Shailaja and Appa Rao. 2018. Comparison of Phenotypic Methods for the Detection of Biofilm Production in Indwelling Medical Devices Used in NICU & PICU in a Tertiary Care Hospital in Hyderabad, India.Int.J.Curr.Microbiol.App.Sci. 7(9): 3265-3273. doi: https://doi.org/10.20546/ijcmas.2018.709.405