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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 3163-3169
DOI: https://doi.org/10.20546/ijcmas.2018.709.394


Incidence and Molecular Characterization of Staphylococcus aureus Isolated from Meat Products
B. J. Shathish Sharma1, S. Rathnapraba1*, S. Meignanalakshmi1, S. Manoharan2
S. Saranya1 and K. Vijayarani1
1Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai - 07, Tamil Nadu, India
2Vaccine Research Centre – Bacterial Vaccine, Centre for Animal Health Studies, TANUVAS, Chennai - 51, Tamil Nadu, India
*Corresponding author
Abstract:

The presence of microorganisms in food is predictable, which causes significant threat to the public health. Staphylococcus aureus is one such foodborne bacterial pathogen that cuses various foodborne outbreaks worldwide. The incidence of S. aureus in food samples is common and it has been characterized as economically important foodborne bacterial pathogens. In the present study, the incidence of S. aureus was investigated in various ready to eat meat products (n=100) purchased from retail market in Chennai. Overall 20% incidence of S. aureus was in different meat products. The prevalence of Staphylococcus aureus in different meat products were 25.6%, 33.3%, 23.8% and 3.7% for chicken, mutton, pork and beef products respectively. All the presumptive colonies of S. aureus was further screened by femA gene specific PCR. The results revealed that out of 20 samples, 11 samples showed positive for femA gene specific PCR. Sequence analysis of S. aureus revealed that all the isolates have 100% homology with the other S. aureus isolates in the NCBI database. All the isolates were screened for their antibiotic resistant pattern. The results revealed that all the isolates showed 100% resistant towards Penicillin, Vancomycin and Methicillin antibiotics. The present study results concluded that, a relatively higher incidence of S. aureus in the meat products marketed in Chennai. Hence, the hygienic quality of the food products needs to be improved to avoid major foodborne outbreaks.


Keywords: Staphylococcus aureus, Meat products, femA, Methicillin resistant and prevalence
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How to cite this article:

Shathish Sharma, B.J., S. Rathnapraba, S. Meignanalakshmi, S. Manoharan, S. Saranya and Vijayarani, K. 2018. Incidence and Molecular Characterization of Staphylococcus aureus Isolated from Meat Products.Int.J.Curr.Microbiol.App.Sci. 7(9): 3163-3169. doi: https://doi.org/10.20546/ijcmas.2018.709.394