International Journal of Current Microbiology and Applied Sciences |
ISSN: 2319-7706 Volume 2 Number 4 (2013) pp. 155-165 |
Comparison of bacterial population in industrial and agricultural soils |
F. Z. Tanu* and S. Hoque |
Department of Soil, Water and Environment, University of Dhaka, Bangladesh, *Corresponding author: tanu_du@yahoo.com |
A B S T R A C T |
This study focused on the isolation, characterization and identification of some
common soil bacterial strains with the aim of investigation of population diversity and comparison of species variation in industrial and agricultural soils. A total of 15 bacteria were isolated from four soil samples collected from Dhaka Export Processing Zone (DEPZ) at Savar (SE), Tannery area at Hajaribagh (ST), Dhaka, and two agricultural fields of Dhamrai (SD) and Kushtia (SK) in Bangladesh. After characterization of the isolates morphologically, physiologically and
biochemically, they were identified provisionally as different species of Bacillus, Micrococcus, and Pseudomonas. For further confirmation by using PCR technique
and 16S rRNA gene sequencing 4 isolates were identified as Micrococcus luteus
strain P4_3 (from SE) and Bacillus megaterium strain H2 (from ST) from industrial soils and Bacillus amyloliquefaciens strain SCSAAB0007 (from SD) and Bacillus
subtilis strain 1320 (from SK) from agricultural soils. The result of this experiment showed no sharp difference in population diversity of the soils collected from
industrial and agricultural soils. Under the above technique the partial 16S rRNA
gene was amplified by polymerase chain reaction (PCR) using gene specific primers (universal primer) and the PCR products were purified and sequenced. Sequence analysis was done using computer based searching procedure through BLAST program. |
Keywords |
Soil bacteria;
industrial soils; agricultural soils; PCR; 16S rRNA
gene sequencing |