International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 3 (2013) pp. 37 43
Optimization of
- Carotene production by Marine
Microalga - Dunaliella salina
D. Selvi Dhanam1 and K. Dhandayuthapani2*
1Research and Development centre, Bharathiar University,
Coimbatore 641 046, Tamil Nadu, India
2Department of Plant Biology and Biotechnology, Arignar Anna Govt. Arts College,
Cheyyar - 604 407, Tamil Nadu, India.
*Corresponding Author: kdpani_bio@yahoo.co.in
A B S T R A C T
Halotolerant microalga Dunaliella sp. is the best commercial source of natural -carotene in the world. In the present investigation, effect of different concentrations of ammonium chloride, sodium phosphate and light intensities on growth and -carotene accumulation by Dunaliella salina were studied. The maximum cell division rate (0.896 day-1) and -carotene accumulation (18μg/106cells) was found to be at 1.0mM/L ammonium chloride. When grown in the different concentrations sodium phosphate the maximum cell division rate (0.947day-1) and -carotene production (24μg/106cells) was noticed at 0.75mM/L. The light intensity 1000 mol/m-2/s-1 with low temperature showed the maximum division rate (0.875 day-1) and -carotene production (21μg/106cells).
Keywords
Ammonium chloride; -carotene; Dunaliella salina; Light intensity; Sodium Phosphate.