International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 3 (2013) pp. 29 36
Evaluation of Indole-3-Acetic Acid in phosphate solubilizing Microbes
isolated from Rhizosphere soil
S. Priya*, T.Panneerselvam1 and T. Sivakumar2
*Department of Microbiology, Sri Akilandeswari Women s College, Vandavasi,
T.V.Malai, Tamil Nadu, India.
1Department of Microbiology, Adiparasakthi College of Arts and Science,
G.B.Nagar, Kalavai 632 506, Tamilnadu, India.
2Department of Microbiology, Kanchi shri Krishna College of Arts & Science,
Kilambi-631551, Kancheepuram, Tamilnadu, India.
*Corresponding author:
Phosphorous is one of the major nutrients needed to dry plants and micro organisms for performing in crop fields. Among micro organisms several bacterial species posses the ability to solubilize tri calcium phosphate. From the Rhizosphere soil many species of phosphate solubilizing bacteria like Bacillus, Pseudomonous sp , Xanthomonas sp. were isolated and fungi like Aspergillus niger, Aspergillus flavus , Aspergillus fumigatus and Penicillium spp. were isolated. Then the PSM solubilizing effects were studied in pikovsyakas medium. Then the PSM isolates were subjected to production of IAA in pikovskayas broth in-vitro condition by adding Ltryptophan as a substrate. Then pH range of Pseudomonous 3sp .was initially at 6.8 and finally decreased to 4.63 then produce more Indole acetic acid. The pH range of Aspergillus niger was initially at 6.8and finally reduced to 4.29 and produce more Indole acetic acid in Fungi. Then finally the IAA was observed in thin layer Chromatography. There is increase evidence that phosphobacteria improve plant growth due to biosynthesis of plant growth substances rather than their action in releasing phosphorous.
Phosphate Solubilizing; Microbes; Tri Calcium Phosphate; Salkowski reagent; TLC.