| International Journal of Current Microbiology and Applied Sciences |
| ISSN: 2319-7706 Volume 2 Number 3 (2013) pp. 19 28 |
| Bacterial extracellular Cholesterol oxidase and its Pharmaceutical perspectives |
| A.D.Bholay*, D.J.Gadekar, S.K.Sawant, and S.M.Sonawane |
| Department of Microbiology, K.T.H.M. College, Nashik, Pune University, MS, India *Corresponding author: adbholay@gmail.com, dhanashree.gadekar@gmail.com |
| A B S T R A C T |
| Cholesterol oxidase is an enzyme which catalyzes the oxidation of cholesterol and converts 5-cholesten-3 -ol into 4-cholesten-3-one. Screening and isolation of bacterial strains producing extracellular form of cholesterol oxidase are very important due to its wide spectrum applications. Cholesterol oxidase enzyme has many applications in medicine, agriculture, industry and pharmaceutical sectors. The various samples- buffalo milk, domestic waste water sludge and fermented fish were used for enrichment and isolation. Out of 10 isolates 6 were cholesterol oxidase positive of which one with significantly high enzyme activity was identified as B. licheniformis. Optimization of physical parameters for enzyme production such as pH and incubation temperature were determined and found to be 7.0 and 350C respectively. Enzyme activity was assayed using o-dianisidin-peroxidase method. Antimicrobial and bioinsecticidal activity of purified enzyme was tested. Also the effect of the enzyme on serum cholesterol was studied. The enzymatic oxidation product of cholesterol in serum sample was characterized by the TLC and found to be 20 -Hydroxycholesterol. |
| Keywords |
| Cholesterol oxidase; 5-cholesten- 3 -ol; 4-choleste n-3-one; Serum cholesterol; Bioinsecticide. |