International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7692 Volume 1 Number 1 (2012) pp.23–28

Optimization of various parameters for the production of glucose oxidase using Aspergillus niger

1N. Yogananth*, 2A. Chanthru and 3D.Sivanesan

1Department of Biotechnology, 2Department of Microbiology, Mohamed Sathak College of
Arts and Science, Chennai, Tamil Nadu - 641048, India
3Faculty of Science, Nova Southeastern University, Florida, USA.
*Corresponding author:



In the present study industrially important fungi (Aspergillus niger) was isolated from soil samples using dilution and plating technique. Aspergillus niger was identified by LCB staining. The isolated strains of A.niger were screened for Glucose oxidase production by plate assay. The high yielding strain of A.niger was isolated and maintained in agar slant as wild type. The strain of A.niger was checked for the Glucose oxidase production under different conditions, like pH, temperature, different carbon sources, nitrogen sources and CaCO3 concentration.  Temperature (60) and pH stability (8) of enzyme were also studied. The strain of A.niger should increase the production of Glucose oxidase  under most of the conditions. Glucose oxidase was characterized by TLC technique and confirmed as Glucose

Keywords : Isolation of A. niger;Assay of GOD enzyme; Characterization of GOD; Optimization of GOD.