International Journal of Current Microbiology and Applied Sciences |
ISSN: 2319-7706 Volume 2 Number 10 (2013) pp. 418-427 |
DNA Based Identification and Characterization of Thermophilic Streptomyces sp. From Desert Soil of Rajasthan |
Dimple Songara* and Swarnjeet Kaur |
Karanja House 2, Dumayne road, Colaba, Mumbai, India |
ABSTRACT |
The present study was aimed at to identify the thermal resistant actinomycetes from
the desert soil of Rajasthan, India. The morphology, cultural, physiological, biochemical and phylogenetic analysis of isolate stb-1 were characterized. According to the morphology, isolate stb-1 was identified as a representative of the genus Streptomyces. The 16S rRNA region of this strain was amplified and sequenced. A Neighbour-Joining algorithm tree of 16S rRNA was constructed. The
strain showed growth at high temperatures and were screened for antagonistic potentiality against gram positive and gram negative human pathogenic bacteria. The strain stb-1 showed broad spectrum antibiotic activity against the tested pathogens. According to the observation and phylogenetic analysis, the strain stb-1
was proved to belong to the genus Streptomyces with thermal resistance and antibiotic potentiality. |
Keywords |
Actinomycetes;
morphology
phylogeny;
antibacterial;
Streptomyces;
thermal
resistance; 16s r RNA |