National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Twenty Five lignolytic fungi were isolated from decaying wood samples and were screened for the laccase production using guaiacol and ABTS as indicator compounds. The positive isolates were confirmed for the presence of laccase using Azure B and quantitative enzyme assay in presence of catalase using ABTS as substrate. Further laccase producing capacity of fungus was estimated in seven different media where Czapex Dox medium showed highest enzymatic activity of 17.2 U/ml. Effect of carbon and nitrogen sources, initial pH, inoculum size, incubation period, incubation temperature, inducer and inhibitor on laccase production was evaluated. The optimal pH, incubation temperature, incubation period and inoculum size for the laccase production in submerged culture were found to be pH 6, 30áµ’C, 7days and 2disc/100ml medium respectively. Sucrose and sodium nitrate were the most suitable carbon and nitrogen sources for laccase production. Out of the different inducers and inhibitors, the prominent induction was observed with 2mM CuSO4 and 0.1% MgSO4 while sodium azide and formaldehyde inhibited the laccase production almost 94% and 74% respectively. Under the optimal condition the maximum laccase activity was determined to be 28.2U/ml which is supposed to be 1.5 times higher than the unoptimized conditions.
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