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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 744-753
DOI: https://doi.org/10.20546/ijcmas.2017.604.092


Assessing Stress Tolerant Rhizobial Isolates of Clusterbean (Cymopsis tetragonoloba (L.) Taub.) Retrieved from Semi- Arid Regions of Haryana, India
Subha Dhull* and Rajesh Gera
Department of Microbiology, CCS Haryana Agricultural University, Hisar-125004, India
*Corresponding author
Abstract:

An attempt has been made to evaluate the effect of abiotic constraints (drought and temperature) on the growth of rhizobia isolated from clusterbean grown in semiarid regions of Haryana with a view to screen out stress tolerant rhizobial isolates. A total of 158 rhizobial isolates have been used for screening their stress tolerating ability with contrast to environmental abiotic soil conditions commonly prevailing in semi-arid regions of Haryana. All the isolates were phenotypically and biochemically characterized. Growth of pure rhizobial isolates on Yeast Extract Mannitol Agar (YEMA) medium having variable range of temperature (30, 35, 40 and 45) and different concentrations of PEG (0, 10, 20, 30, 40 and 50 %) were recorded at 420 nm using UV-VIS spectrophotometer after incubation at 30â—¦C for five days. On the basis of comparison of growth under varied stress conditions, fifty four rhizobial isolates from combined (temperature as well as drought) stress study were screened out. The stress tolerant traits of these rhizobia are of potential value from the point of view of biofertilization of legume seedlings during a forestation of degraded areas in semi-arid tropics of Haryana.


Keywords: Rhizobia, Clusterbean, Drought tolerance, PEG.
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How to cite this article:

Subha Dhull and Rajesh Gera. 2017. Assessing Stress Tolerant Rhizobial Isolates of Clusterbean (Cymopsis tetragonoloba (L.) Taub.) Retrieved from Semi- Arid Regions of Haryana, IndiaInt.J.Curr.Microbiol.App.Sci. 6(4): 744-753. doi: https://doi.org/10.20546/ijcmas.2017.604.092