National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Urinary tract Infections caused by extended-spectrum β-lactamase (ESBL), producing Klebsiella pneumoniae are on a rise all over the world with high morbidity and mortality. This study was carried out to determine the presence of TEM, SHV and CTX-M genes in extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae. A total of 300 Klebsiella pneumoniae isolates were collected and identified using traditional culturing and biochemical tests. Antibiotic susceptibility testing was performed by disc-diffusion method according to the CLSI guideline. Isolates were screened for ESBL and confirmed by phenotypic confirmatory disc diffusion test (PCDDT). 100 randomly selected isolates tested for the presence of ESBL encoding genes using PCR with specific primers for the detection of CTX-M, SHV and TEM genes using a standard protocol. Imipenem showed the highest antibacterial activity against ESBL producing K. pneumoniae. Based on the results of PCR, the prevalence of TEM, SHV and CTX-M genes among ESBLs-positive isolates was 74%, 27%, and 44% respectively. In conclusion, the rate of ESBL-producing K. pneumoniae was high in the present study. The bacterial resistance to many classes of antibiotic leads to limited treatment options. Since the management of infections caused by these organisms is difficult, it is important to control such strains in order to prevent and reduce their spread.
Citations |
 |
 |
 |
 |
