National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Among all the micro nutrient deficiencies, World Health Organization (WHO) had identified Vitamin A deficiency as the more dangerous and widespread micronutrient deficiencies in the world. In order to address this global problem, biofortification of Maize is considered to be a cost effective and sustainable approach. The present study was carried out to raise and identify high β- carotene maize lines of BC1F2 (UMI 1200 × HP 467-15)-C15-S79 population by phenotyping using HPLC along with genotyping of crtRB1 allele. The randomly selected 10 seeds of selected BC1F2 cob were analyzed for β-carotene content through HPLC and the results showed confirmation of improvement in β-carotene content of 3.00 µg/g respectively. The crtRB1 allele polymorphism in BC1F2 (UMI 1200 × HP 467-15)-C15-S79 population of the two crosses revealed that the progenies showed segregation distortion (SD) populations, indicating that the favorable allele is under-represented in the population. The variability studies in BC1F2 population revealed that, low and moderate GCV and PCV was found for all traits which gives an indication of justifiable variability among the genotypes with respect to these characters and therefore gives scope for improvement of these traits in future generations through selection. The PCV was higher than the corresponding GCV for most of the traits under study which results in high heritability. The heritability estimates of BC1F2 generation were found to be highest for plant height. Frequency distribution study of BC1F2 population states that for the traits like plant height, ear height and 100 kernel weight, most of the progenies exhibited the values same as or near to recurrent parent. BC1F2 population, showed positive skewness for days to 50 per cent tasseling, plant height and ear height while negative skewness was observed for leaf length, days to 50 per cent silking and 100 kernel weight. Negative kurtosis was observed for all the six traits, in BC1F2 population. The study indicated that it is possible to develop a high β-carotene inbred with genetic identity closer to that of the recurrent parent in latter generations.
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