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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
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Int.J.Curr.Microbiol.App.Sci.2017.6(4): 270-287
DOI: https://doi.org/10.20546/ijcmas.2017.604.031


Molecular Breeding and Variability Analysis of High Beta Carotene Introgressed BC1F2 Line of Maize
Pitambara1*, P. Nagarajan2 and N. Senthil2
1Department of Agricultural Biotechnology, Anand Agricultural University,Anand-388 110, Gujarat, India
2Department of Agricultural Biotechnology, Tamil Nadu Agricultural University,Coimbatore-641003, India
*Corresponding author
Abstract:

Among all the micro nutrient deficiencies, World Health Organization (WHO) had identified Vitamin A deficiency as the more dangerous and widespread micronutrient deficiencies in the world. In order to address this global problem, biofortification of Maize is considered to be a cost effective and sustainable approach. The present study was carried out to raise and identify high β- carotene maize lines of BC1F2 (UMI 1200 × HP 467-15)-C15-S79 population by phenotyping using HPLC along with genotyping of crtRB1 allele. The randomly selected 10 seeds of selected BC1F2 cob were analyzed for β-carotene content through HPLC and the results showed confirmation of improvement in β-carotene content of 3.00 µg/g respectively. The crtRB1 allele polymorphism in BC1F2 (UMI 1200 × HP 467-15)-C15-S79 population of the two crosses revealed that the progenies showed segregation distortion (SD) populations, indicating that the favorable allele is under-represented in the population. The variability studies in BC1F2 population revealed that, low and moderate GCV and PCV was found for all traits which gives an indication of justifiable variability among the genotypes with respect to these characters and therefore gives scope for improvement of these traits in future generations through selection. The PCV was higher than the corresponding GCV for most of the traits under study which results in high heritability. The heritability estimates of BC1F2 generation were found to be highest for plant height. Frequency distribution study of BC1F2 population states that for the traits like plant height, ear height and 100 kernel weight, most of the progenies exhibited the values same as or near to recurrent parent. BC1F2 population, showed positive skewness for days to 50 per cent tasseling, plant height and ear height while negative skewness was observed for leaf length, days to 50 per cent silking and 100 kernel weight. Negative kurtosis was observed for all the six traits, in BC1F2 population. The study indicated that it is possible to develop a high β-carotene inbred with genetic identity closer to that of the recurrent parent in latter generations.


Keywords: Maize, Beta carotene, Variability, Frequency distribution.
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How to cite this article:

Pitambara, P. Nagaraja and Senthil, N. 2017. Molecular Breeding and Variability Analysis of High Beta Carotene Introgressed BC1F2 Line of Maize.Int.J.Curr.Microbiol.App.Sci. 6(4): 270-287. doi: https://doi.org/10.20546/ijcmas.2017.604.031