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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 253-264
DOI: https://doi.org/10.20546/ijcmas.2017.604.029


Biochemical, Nutritional Profiling and Optimization of an Efficient Nucleic Acid Isolation Protocol from Recalcitrant Tissue of Wild Edible Fruit Antidesma bunius L. Spreng
Sushma Khomdram1,2*, Shyamananda Arambam2,Sharmistha Barthakur2 and Guruaribam Shantibala Devi1
1Department of Life Sciences, Manipur University, Canchipur 795003, Manipur, India
2National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi 110012, India
*Corresponding author
Abstract:

Antidesma bunius known as ‘currant tree’ is a popular fruit in several Asian countries. During our exploration of wild fruits of Manipur, India, we identified this edible fruit growing naturally in their wild habitat. An exhaustive biochemical analyses revealed very high concentration of mineral contents relative to other well known fruits. Correlation analysis showed significant relationship between ascorbic acid content and antioxidant oxidant activity. A protocol for isolation of high quality DNA from mature leaves of the fruit tree which is very rich in polyphenols and secondary metabolites was also standardized for the very first time with high purity and quality genomic DNA amenable to downstream initiation of any molecular work in this species. Our results bring further potential and necessity of popularizing this fruit amongst general consumers.


Keywords: Biochemical analysis, DNA, Antidesma bunius, Wild fruit.
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How to cite this article:

Sushma Khomdram, Shyamananda Arambam, Sharmistha Barthakur and Guruaribam Shantibala Devi. 2017. Biochemical, Nutritional Profiling and Optimization of an Efficient Nucleic Acid Isolation Protocol from Recalcitrant Tissue of Wild Edible Fruit Antidesma bunius L. spreng.Int.J.Curr.Microbiol.App.Sci. 6(4): 253-264. doi: https://doi.org/10.20546/ijcmas.2017.604.029