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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 174-181
DOI: https://doi.org/10.20546/ijcmas.2017.604.020


Resazurin Microplate Assay: Rapid Assay for Detection of Methicillin Resistant Staphylococcus aureus
Hala Mahmoud Hafez1, Dalia H Abd El Hamid1*, Dina Tarek1 and Fatma Al-Zahraa M. Gomaa2
1 Department of Clinical Pathology, Faculty of Medicine, Ain Shams University, Egypt
2Department of Microbiology, Faculty of Pharmacy, Al-Azhar University for Girls, Egypt
*Corresponding author
Abstract:

The increasing methicillin resistant Staphylococcus aureus (MRSA) infections pose a serious threat. Accurate and rapid detection of methicillin resistance is important for ensuring the prompt start of antibiotherapy and control of MRSA in the hospitals. Molecular detection of mecA gene is the gold standard for identification of MRSA isolates. However, many laboratories don’t have the capacity for molecular techniques. Resazurin is a dye used as an oxidation reduction indicator in bacterial cell viability assays. The aim of the study was to introduce resazurin microplate assay (REMA) as a new colorimetric method for identification of MRSA. The study included 100 Staph. aureus clinical isolates which were tested for their susceptibility to methicillin by the cefoxitin (30ug) disc diffusion (DD) method and REMA. Detection of the mecA gene was done by PCR. Out of the 100 studied isolates, 65% were MRSA by DD and the REMA. A highly significant association was found between the results of DD method and the results of REMA. The mecA gene was detected in 64/65 of the MRSA isolates. There was a highly significant association between the results of REMA and that of the PCR for the mecA gene. It is concluded that the REMA is a sensitive and specific assay for rapid phenotypic detection of MRSA in poor resource laboratories.


Keywords: MRSA, Resazurin, REMA, mecA gene, Cefoxitin.
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How to cite this article:

Hala Mahmoud Hafez, Dalia H Abd El Hamid, Dina Tarek and Fatma Al-Zahraa M Gomaa. 2017. Resazurin Microplate Assay: Rapid Assay for Detection of Methicillin Resistant Staphylococcus aureus.Int.J.Curr.Microbiol.App.Sci. 6(4): 174-181. doi: https://doi.org/10.20546/ijcmas.2017.604.020