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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 1655-1675
DOI: https://doi.org/10.20546/ijcmas.2017.603.192


Identification and Characterization of Bile Salt Hydrolyzing Lactobacillus Isolates
Pradip Kumar Sharma1, Pradeep Kumar Sharma1 and Naresh Kumar2, Suman2 and Niti Dhingra3
1Department of Microbiology, Ch. Charan Singh University Meerut, India
2Dairy Microbiology Division, National Dairy Research Institute, Karnal, India
3Department of Biotechnology, DAV College, Sec, 10, Chandigarh, India
*Corresponding author
Abstract:

Thirty nine, out of 46 acid tolerant Lactobacillus spp., isolated from traditional dairy products, fermented foods and human fecal samples, were preliminary identified at genus level as Lactobacillus and evaluated for probiotic properties which included bile tolerance, simulated gastrointestinal juice, cell surface hydrophobicity, cell auto-aggregation, co-aggregation and bile salts hydrolase activity. Thirty three isolates could resist well at 1.5 % bile while 26 isolates showed tolerance towards simulated gastric juice (pH 2.0, 3 h of incubation) and simulated pancreatic juice (pH 8.0). Only 9 isolates could exhibited >50% cell surface hydrophobicity wherein LBF89, LBF91 and LBF90 exhibited highest cell surface hydrophobicitywith n-hexadecane i.e. 73.47±2.15 %, 72.25±1.69 % and 71.65±1.90 % respectively. LBF89 showed highest cell auto-aggregation (50.67±1.08%) while least auto-aggregation was demonstrated by LBF 11. LBF 20 exhibited highest mean co-aggregation 50.17 % with E. coli, L. monocytogenes, S. abony and S. aureus. S. abony was the highly encountered pathogen with an average value of 31.14 % ofco-aggregation. Highest bile salt hydrolase activity was observed in LBF 89 and LBF 91with sodium taurodeoxycholate. Bile salt hydrolase activity was quantitatively determined wherein the highest activity i.e.7.21±0.10, was observed in LBF 89 isolate followed by LBF 91 with 6.56±0.10 as total enzyme activity. Both the isolates were identified using PCR as Lactobacillus plantarum but distantly placed in the phylogenetic tree. Among the selected isolates, LBF 89 and LBF 91 showed the best probiotic potential with high tolerance to bile, simulated gastrointestinal juice and exhibited high bile salt hydrolase activity. Both the isolates possess application potential for functional foods and health-associated products.


Keywords: Probiotic, Lactobacillus, BSH activity, Molecular identification.
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How to cite this article:

Pradip Kumar Sharma, Pradeep Kumar Sharma and Naresh Kumar, Suman and Niti Dhingra. 2017. Identification and Characterization of Bile Salt Hydrolyzing Lactobacillus Isolates.Int.J.Curr.Microbiol.App.Sci. 6(3): 1655-1675. doi: https://doi.org/10.20546/ijcmas.2017.603.192