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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 2152-2162
DOI: https://doi.org/10.20546/ijcmas.2017.603.246


Antimicrobial Activity and Phytochemical Screening of Aloe vera (Aloe barbadensis Miller)
Darshan Dharajiya1*, Nalin Pagi2, Hitesh Jasani3 and Payal Patel4
1Department of Plant Molecular Biology and Biotechnology, C. P. College of Agriculture, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar-385506, Gujarat, India
2Department of Genetics and Plant Breeding, CPCA, SDAU, Sardarkrushinagar-385506, Gujarat, India
3Department of Microbiology, College of Computer, Science and Information Technology (CCSIT), Junagadh-362015, Gujarat, India
4Department of Biotechnology, ARIBAS, New V.V.Nagar-388121, Gujarat, India
*Corresponding author
Abstract:

The present study was conducted to assess the antimicrobial potential and phytochemical analysis of Aloe vera (Aloe barbadensis Miller) leaves extracts.The extracts were prepared by the sequential cold maceration method by using hexane, ethyl acetate, methanol and distilled water as a solvent. Antimicrobial activity of four extracts was performed by agar well diffusion method against different bacteria and fungi. Determination of Minimum Inhibitory Concentration (MIC) of different extracts, Thin Layer Chromatography (TLC), TLC bioautography and qualitative phytochemical analysis were also performed.The antimicrobial activity of A. barbadensis leaves extracts was found maximum against S. marcescens with a Zone of Inhibition (ZOI) of 13.67±0.57mm by hexane extract.The MIC of different extracts ranged between 6.25 and 50.00 mg/ml. Among all the fungi used in the study, all the three Aspergillus species were slightly inhibited by the specific extracts. The finding of TLC bioautography showed that compounds eluted at Rf 0.65 demonstrated strong antimicrobial activity whereas compounds eluted at Rf0.41 and Rf 0.82 exhibited moderate antimicrobial activity against S. marcescens. Phytochemical analysis indicated the presence of phytochemicals present in various extracts. The results of the investigation clearly indicate that A. barbadensis leaves extract have a potential antimicrobial activity against various microorganisms due to the presence of various phytochemicals.
 


Keywords: Antimicrobial activity, Aloe vera, A. barbadensis Miller, TLC Bioautography, Phytochemical analysis.
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How to cite this article:

Darshan Dharajiya, Nalin Pagi, Hitesh Jasani, Payal Patel. 2017. Antimicrobial Activity and Phytochemical Screening of Aloe vera (Aloe barbadensis Miller).Int.J.Curr.Microbiol.App.Sci. 6(3): 2152-2162. doi: https://doi.org/10.20546/ijcmas.2017.603.246