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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 1364-1373
DOI: https://doi.org/10.20546/ijcmas.2017.603.158


Isolation and screening of nitrogen fixing endophytic bacterium Gluconacetobacter diazotrophicus GdS25
Hema C. Rao* and V.P. Savalgi
Department of Agricultural Microbiology, University of Agricultural Sciences, Dharwad – 580005, Karnataka, India
*Corresponding author
Abstract:

The present study is on the isolation, characterization and screening of endophytic bacterium i.e., Gluconacetobacter diazotrophicus which was isolated from the tissues of surface sterilized roots of four different crops viz., sugarcane, maize, pineapple and carrot. The morphological and biochemical tests permitted characterization of Gluconacetobacter diazotrophicus isolates. Further the plant growth promoting traits such as nitrogen fixing ability, production of phytohormones (Indole acetic acid and Gibberellic acid), phosphorus and zinc solubilization and antagonistic activity by dual culture method were assessed. Out of 113 isolated screened only 10 efficient isolates from various crops were selected based on nitrogen fixing capacity. An isolate from sugarcane GdS25 fixed maximum nitrogen i.e., 147µg of N/mg of Carbon used and produced maximum concentration of Indole acetic acid (4.94µg/ml) and Gibberellic acid (7.1µg/25ml) respectively. GdS25 showed phosphorus solubilization zone of hydrolosis 2.1cm where as zone of zinc solubilisation of 3.5cm. An isolate from sugarcane GdS25 exhibited 83% inhibition against Rhizoctonia bataticola and 88% against Exerohilium maydis. The GdS25 is more potent as compared to the reference strain G. diazotrophicus MTCC1224.Five efficient isolates viz., GdS25, GdS26, GdM5, GdM6 and GdP7 will be taken up for further studies.


Keywords: Gluconacetobacter diazotrophicus, Nitrogen fixing ability, Phytohormones, Phosphorus and Zinc solubilization.
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How to cite this article:

Hema C. Rao and Savalgi, V.P. 2017. Isolation and screening of nitrogen fixing endophytic bacterium Gluconacetobacter diazotrophicus GdS25Int.J.Curr.Microbiol.App.Sci. 6(3): 1364-1373. doi: https://doi.org/10.20546/ijcmas.2017.603.158