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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 1259-1269
DOI: https://doi.org/10.20546/ijcmas.2017.603.145


Lipid Screening, Extraction and Production of Biodiesel from Marine Diatom Odontella aurita
Shailesh Upadhyay1*, Kapil Lawrence1, Sudhir K. Shekhar2, P. Anantharaman3
1Department of Biochemistry and Biochemical Engineering, JSBB, SHIATS, Allahabad, India
2Department of Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Lucknow, India
3CAS in Marine Biology, Annamalai University, Parangipettai, Tamilnadu, India
*Corresponding author
Abstract:

The marine diatom based biodiesel may be the greatest possible options for future energy, particularly for India where land resources and freshwater are limited. In the above context, an attempt has been made to isolate the marine diatom Odontella aurita from Vellar estuary, South east coast of India and characterized for biodiesel production. Identification of microscopic structure, morphology was succeeding by bright field microscope and SEM. In the present study, the effect of different Si treatment on the growth and lipid content confirmed that the cell density is maximum at 159 µM Si medium 10.21±0.16×105 cells ml-1 and minimum at 0 µM Si medium (medium without Si) 5.5±0.10×105 cells ml-1, accumulation of lipid content maximum at 53 µM Si medium 23.7± 79% of dry biomass were minimum 13.25± 76% of dry biomass at 159 µM Si medium. Fatty Acid Methyl Esters (FAME) produced by transesterification of lipid obtained at 53 µM Si medium Results show that among the fatty acids, saturated fatty acid (SFAs) was found to be 43.61% followed by monounsaturated fatty acid (33.22%) and polyunsaturated fatty acid (23.17%). From the above results, it is concluded that O. aurita species having low percentage of polyunsaturated fatty acids and higher percentage of saturated fatty acid that could be suitable for further exploration and commercial biodiesel production.


Keywords: Odontella aurita, Lipid, Si, Transesterification, GC-MS,Biodiesel and SEM
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How to cite this article:

Shailesh Upadhyay, Kapil Lawrence, Sudhir K. Shekhar, Anantharaman, P. 2017. Lipid Screening, Extraction and Production of Biodiesel from Marine Diatom Odontella aurita.Int.J.Curr.Microbiol.App.Sci. 6(3): 1259-1269. doi: https://doi.org/10.20546/ijcmas.2017.603.145