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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 919-928
DOI: https://doi.org/10.20546/ijcmas.2017.603.109


Isolation, Production and Optimization of Siderophores (Iron Chilators) from Pseudomonas fluorescence NCIM 5096 and Pseudomonas from Soil Rhizosphere and Marine Water
Jayashri Pattan1*, Swapnil Kajale2 and Shashikant Pattan3
1Dr D.Y. Patil Arts, Commerce and Science College, Pimpri, Pune, India, 411018
2Microbial Culture Collection, National Centre for Cell science, Pune, India 411021
3Abasaheb Kakade Collage of B. Pharmacy, Bodhegaon, A’nagar, India
*Corresponding author
Abstract:

Two Pseudomonas spp. are isolated from different natural sources i.e. soil rhizosphere and marine water on selective media like cetrimide agar base and King’s B medium. Pseudomonas fluorescence NCIM 5096 and other two isolated of Pseudomonas produced siderophore with the yield 71%, 72.33%, 33% of hydroxamate type, in iron free succinate medium (SM). Nutrient medium inhibited the growth of siderophore while the succinate medium gave the maximum production of siderophore. pH has important role in production of siderophore i.e. at neutral pH 7; maximum siderophore yield while at acidic pH production was found ceasing. Succinate medium supplemented with Pb showed enhancement while other supplemented with Co showed inhibition of production of siderophore. Isolated siderophore showed the antagonists effects against human pathogenic Pseudomonas aeruginosa and on phytopathogenic fungi.


Keywords: Siderophores, Phytopathogens,Pseudomonas sp.
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How to cite this article:

Jayashri Pattan, Swapnil Kajale, Shashikant Pattan. 2017. Isolation, Production and Optimization of Siderophores (Iron Chilators) from Pseudomonas fluorescence NCIM 5096 and Pseudomonas from Soil Rhizosphere and Marine Water.Int.J.Curr.Microbiol.App.Sci. 6(3): 919-928. doi: https://doi.org/10.20546/ijcmas.2017.603.109