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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 687-693
DOI: https://doi.org/10.20546/ijcmas.2017.603.079


Comparison of Staining of Keratin Pearl and Individual Cell Keratin in Oral Squamous Cell Carcinoma by Modified Papanicolaou,Modified Mallory’s and Hematoxylin and Eosin Stain
Santhosh Kumar Caliaperoumal1*, Sudhakar Srinivasan2 and Meer Ahmed Ibrahim Munshi3
1Department of Dentistry, Vinayaka Mission’s Medical college and Hospital,Karaikal, Puducherry, India
2Department of Dentistry, Karpagam Medical College and hospital, Coimbatore,Tamil nadu, India
3Department of Oral surgery, Sri Ramakrishna dental college and hospital, Coimbatore, Tamilnadu, India
*Corresponding author
Abstract:

Observation of Keratin pearl (KP) and individual cell keratin (ICK) is one of the criteria in grading oral squamous cell carcinoma (SCC). The aim of the study was to evaluate and compare the distinct staining and identification of KP and ICK by routine Hematoxylin and Eosin (H&E) stain Modified Mallory’s and Modified Papanicolaou Stain. A total number of 38 paraffin embedded tissues of known cases of well-differentiated and moderately-differentiated SCC were taken and 3 sections of 4-5 micron thickness from each block were cut and stained with H&E stain, Modified Mallory’s and modified Papanicolou stain. The KP and ICK were distinctly and clearly stained by modified Papanicolou, modified Mallory’s stain compared to H&E stain. The positive staining of KP and ICK by modified Papanicolou stain was statistically significant than modified Mallory’s and H&E stain at P=0.033 and P=0.001. Based on our findings we conclude that the efficacy of distinct identification of KP and ICK in oral SCC by modified Papanicolou stain is better than modified Mallory’s stain and H&E stain. So it can be used as an adjuvant in case of oral SCC.


Keywords: Squamous cell carcinoma (SCC),Keratin Pearl (KP), Individual cell Keratin (ICK), Modified Papanicolou (PAP) stain, Hematoxylin and Eosin (H&E) stain.
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How to cite this article:

Santhosh Kumar Caliaperoumal, Sudhakar Srinivasan, Meer Ahmed Ibrahim Munshi. 2017. Comparison of Staining of Keratin Pearl and Individual Cell Keratin in Oral Squamous Cell Carcinoma by Modified Papanicolaou, Modified Mallory’s and Hematoxylin & Eosin Stain.Int.J.Curr.Microbiol.App.Sci. 6(3): 687-693. doi: https://doi.org/10.20546/ijcmas.2017.603.079