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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 1-12
DOI: https://doi.org/10.20546/ijcmas.2017.603.001


Antimicrobial Activity in vitro of Aqueous Extracts of Oregano (Origanum vulgare L.) and Thyme (Thymus vulgaris L.)
Ralitsa Bankova and Teodora P. Popova*
University of Forestry, Faculty of Veterinary Medicine, 10 Kl. Ohridski Blvd.,Sofia 1756, Bulgaria
*Corresponding author
Abstract:

The antimicrobial effect of hot and cold water extracts and infusions of herbs oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.), prepared in concentrations of 20%, was investigated using the classical agar-gel diffusion method. Pure cultures of 20 pathogenic strains were tested (by 4 strains of Pseudomonas aeruginosa, Klebsiella pneumoniae, Pasteurella multocida, Enterococcus faecalis and Candida albicans). The highest antibacterial and antimycotic effect in vitro exhibited the infusions of both herbs, similar to that of the control broad-spectrum antibiotic thiamphenicol. The inhibitory effect of the infusion of thyme was slightly higher than that of the infusion of oregano. Highest sensitivity to both infusions showed the tested strains of P. aeruginosa and C. albicans, and the lowest - these of P. multocida. The cold water extracts of both studied herbs showed less pronounced antimicrobial effect, which was slightly higher in thyme. The cold extract of oregano showed pronounced antibacterial effect against the strains of P. aeruginosa and K. pneumoniae, but not to those of P. multocida. The hot aqueous extracts of the two herbs manifested weakest antimicrobial activity in vitro. The inhibitory effect of hot water extract of oregano was slightly higher than that of thyme.


Keywords: Origanum vulgare, Thymus vulgaris, Water extracts, Antimicrobial activity.
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How to cite this article:

Ralitsa Bankova and Teodora P. Popova. 2017. Antimicrobial Activity in vitro of Aqueous Extracts of Oregano (Origanum vulgare L.) and Thyme (Thymus vulgaris L.).Int.J.Curr.Microbiol.App.Sci. 6(3): 1-12. doi: https://doi.org/10.20546/ijcmas.2017.603.001